No downloads, no notes for slide. Jessica Olivares 11/22/2014, bio 3103, fly lab report, fruit Fly. Report, hypothesis, in setting up the fruit fly experiment the predicted mutant phenotypes were to be compared in the observed F1 generation and F2 generation. In order to make the appropriate phenotypic ratios we based the F1 and F2 to generate two mutants that were used to set up the parental cross. In the observed F1 generation the white eye mutant was located on the male drosophila melanogaster. The following white eye mutation could be set up by the following white type white eye female and a combination of wild type male with vestigial wings.
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There is no guarantee, due to randomness of the system, that there will be a ratio that will ever exactly mimic real life scenarios parks in nature. In both experiments the Chi-Square figure showed that both null hypotheses were rejected. These numbers were not comparably close to those needed to have had an accepted null hypothesis. The fertility of these fruit flies, the frequency of mating, the genes that were involved with crossing over, and the chromosomes that were passed on to the next generation(s) were all due to random assortment and therefore it could only be just a possibility that. Mutations can also influence such ratios with traits that were not accounted for in the past and are consequently passed on to future generations. Retrieved October 5, 2012 from:. The Fly people make history. The genes we share. Fruit elegant Fly genetics lab report, successfully reported this slideshow. Fruit Fly genetics lab report, upcoming SlideShare. Loading in 5, be the first to like this.
Sex-Linked Cross: The P1 genotype and phenotypes consisted of: XwXw white-eyed females and Xwy red-eye males. Six of each was crossed and the F1 generation was shown to have consisted of: XwXw red-eye females and XwY white-eye males. The expected ratio was 1:1 red to white. With business a total count of 357 fruit flies, the observed number of red-eye females was 220 while the observed number of white-eye males was 137 which did not fit the expected ratio. The Chi-Square for our group was found to.3 which was too high to be accepted, thus the null hypothesis was found to be rejected. The Chi-Square of the class was found to.0 and once again too high to be accepted, thus the null hypothesis was rejected. Conclusion, the results of both of these experiments were similar in terms of the acceptance or rejecting of the null hypothesis. The expected probability ratios were theoretically supposed to have closely mimicked the experimental results; however probability is just that, probable.
Monohybrid Cross: The total observed numbers counted was 358 with 333 (93) of them having the red-eye phenotype and a genotype(s) of homozygous really rr and/or heterozygous. The remaining fruit 25 (7) were of the sepia-eye colored phenotype with a definite genotype of homozygous. The expected ratio of the null hypothesis was 3:1 (75) red-eye colored and 1:3 (25) sepia-eye colored or expected numbers of 268.5 (red) and.5 (sepia). P1 eye genotype the Chi-Square calculations were figured by taking the standard Chi-Square computations as shown in Table. The Chi-Square number for our experiment was.0. This number was significant in comparison to what would have been needed for the null hypothesis to have been accepted, thus enabling the conclusion of a rejected hypothesis. The class as a whole also calculated the Chi-Square by adding up all the observed numbers and expected numbers to form a class total. This figure was significantly larger at a chi-Square of 206.7, also leading to a rejected null hypothesis.
Adult flies of the F1 generation were visible after a week. These adult flies were anesthetized and the phenotypes (sex and eye color) were observed and recorded. A chi-Square statistical analysis was calculated to determine if the null hypothesis of 1:1 for fly sex and eye color would be accepted or rejected. This data was combined with data from the entire class to increase the sample population and a second Chi-Square was calculated. Results, the P1 Generation for the monohybrid cross consisted of six homozygous red-eye (RR) fruit flies of one sex and six homozygous sepia (rr) fruit flies of the other sex. As shown in Table 1 the F1 pairing consisted of being all heterozygous for the wild-type red-eye (Rr) in coloration. The F2 generation is also presented showing an unexpected ratio and the Chi-square is calculated in Table. The P1 generation of the sex-linked inheritance started with white-eyed females and red-eyed males and the results in the F1 progeny are presented in Table 3 while the Chi-square is illustrated in Table.
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Be sure to writing follow your institutions guiding procedures for Lab Report Writing so that it can be up to be up to standard. 11, 2012 a new culture bottle with fresh media and yeast was prepared and labeled. Six male flies and six female flies from the F1 generation were added to the new culture bottle. Once larvae and pupae became visible the adult F1 generation fruit flies were removed from the culture leaving behind the F2 generation offspring. Seven to ten days later the offspring had developed into adult flies. The F2 generation was anesthetized and then separated and counted based on eye phenotype which was either red or doing sepia. The numbers observed were used to calculate a chi-Square statistical analysis which was used to determine whether or not the null hypothesis of a 3:1 ratio for the F2 generation was accepted.
This data was combined with data from the entire class to increase the sample population and to calculate another Chi-Square. Sex-linked Cross: A culture bottle with, drosophila media and yeast was prepared and labeled. Melanogaster wild-type male flies and six white-eyed female flies were transferred into the bottle. Seven to ten days later numerous larvae and some pupae became visible inside the bottle. At this time the P1 generation flies were removed from the bottle leaving only the F1 generation progeny as to avoid intergenerational mating.
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